Vývoj a validácia HPLC metódy pre stanovenie bioaktívnych látok v rastlinných extraktoch

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Vývoj a validácia HPLC metódy pre stanovenie bioaktívnych látok v rastlinných extraktoch

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dc.contributor.advisor Kašpárková, Věra
dc.contributor.author Hanúsková, Veronika
dc.date.accessioned 2016-11-28T12:38:03Z
dc.date.available 2016-11-28T12:38:03Z
dc.date.issued 2016-01-20
dc.identifier Elektronický archiv Knihovny UTB
dc.identifier.uri http://hdl.handle.net/10563/39038
dc.description.abstract Táto diplomová práca sa zameriava na vývoj HPLC metódy pre stanovenie vybraných bioaktívnych látok z rastliných extraktov. Presnejšie sa práca zaoberá separáciou vybraných flavonoidov a to rutínu, hesperidínu, naringínu, fenolpropanoidov: dihydrokumarínu, eskulínu a escínu a derivátom antrachinónu hypericínu. Tieto látky pochádzajú z rastlín: Pagaštan konský (Aesculus hippocastanum), Pohánka tatárska (Fagopyrum tataricum (L.)), Komunica lekárska (Melilotus officinalis (L.)), Ľubovník bodkovaný (Hypericum perforatum) a Grep (Citrus paradisi). Pre toto stanovenie boli vyvinuté tri metódy. Metóda s gradientom bola použitá pre stanovenie rutínu, hesperidínu, naringínu, dihydrokumarínu a eskulínu. Escín a hypericín boli potom analyzované pomocou izokratických metód.
dc.format 99
dc.language.iso cs
dc.publisher Univerzita Tomáše Bati ve Zlíně
dc.rights Bez omezení
dc.subject rutín cs
dc.subject hesperidín cs
dc.subject naringín cs
dc.subject dihydokumarín cs
dc.subject escín cs
dc.subject hypericín cs
dc.subject Aesculus hippocastanum cs
dc.subject Fagopyrum tataricum cs
dc.subject L. cs
dc.subject Melilotus officinalis cs
dc.subject L. cs
dc.subject Hypericum perforatum a Citrus paradisi cs
dc.subject rutin en
dc.subject hesperidin en
dc.subject naringin en
dc.subject dihydrocoumarin en
dc.subject escin en
dc.subject hypericin en
dc.subject Aesculus hippocastanum en
dc.subject Fagopyrum tataricum en
dc.subject L. en
dc.subject Melilotus officinalis en
dc.subject L. en
dc.subject Hypericum perforatum and Citrus pa-radisi en
dc.title Vývoj a validácia HPLC metódy pre stanovenie bioaktívnych látok v rastlinných extraktoch
dc.title.alternative Development and Validation of HPLC Method for Determining of Bioactive Substances in Plant Extracts
dc.type diplomová práce cs
dc.contributor.referee Krejčí, Jiří
dc.date.accepted 2016-06-08
dc.description.abstract-translated This diploma thesis aims at the development of the HPLC method for identification and quntification of chosen bioactive substances from herbal extracts. More specifically, the thesis deals with the separation of particular flavonoids (rutin, hesperidin, naringin), phenylpropanoids (dihydrocoumarin, aesculin, aescin) and anthraquinone derivatives (hypericin). These substances originate from the following herbs: European horse-chestnut (Aesculus hippocastanum), tartary buckwheat (Fagopyrum tataricum (L.)), yellow sweet clover (Melilotus officinalis (L.)), St John's wort (Hypericum perforatum) and grapefruit (Citrus paradisi). For the above-mentioned determination, three methods were developed. The gradient method was used in the identification and quantification of rutin, hesperidin, naringin, dihydrocoumarin and aesculin. Aescin and hypericin were then analyzed by means of isocratic methods.
dc.description.department Ústav technologie tuků, tenzidů a kosmetiky
dc.thesis.degree-discipline Technologie tuků, detergentů a kosmetiky cs
dc.thesis.degree-discipline Technology of Fat, Detergent and Cosmetics Production en
dc.thesis.degree-grantor Univerzita Tomáše Bati ve Zlíně. Fakulta technologická cs
dc.thesis.degree-grantor Tomas Bata University in Zlín. Faculty of Technology en
dc.thesis.degree-name Ing.
dc.thesis.degree-program Chemie a technologie potravin cs
dc.thesis.degree-program Chemistry and Food Technologies en
dc.identifier.stag 44297
utb.result.grade A
dc.date.submitted 2016-05-17


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